Onder andere met: Ben Stiller (Larry Daley), Dick Van Dyke (Cecil), Mickey Rooney (Gus), Jake Cherry (Nick Daley), Bill Cobbs (Reginald), Ricky Gervais (Dr. McPhee), Carla Gugino (Rebecca), Mizuo Peck (Sacajawea), Robin Williams (Teddy Roosevelt), Kim Raver (Erica Daley), Patrick Gallagher (Atilla the Hun), Rami Malek (Ahkmenrah), Pierfrancesco Favino (Christopher Columbus), Steve Coogan (Octavius), Owen Wilson (Jedediah)