Onder andere met: Andrew Garfield (Spider-Man / Peter Parker), Emma Stone (Gwen Stacy), Jamie Foxx (Electro / Max Dillon), Dane DeHaan (Green Goblin / Harry Osborn), Colm Feore (Donald Menken), Felicity Jones (Felicia), Paul Giamatti (Aleksei Sytsevich), Sally Field (Aunt May), Embeth Davidtz (Mary Parker), Campbell Scott (Richard Parker), Marton Csokas (Dr. Ashley Kafka), B.J. Novak (Alistair Smythe), Sarah Gadon (Kari), Michael Massee (Gustav Fiers), Jorge Vega (Jorge)