Onder andere met: Sandra Bullock (Scarlet Overkill (voice)), Jon Hamm ( Herb Overkill (voice)), Michael Keaton (Walter Nelson (voice)), Allison Janney (Madge Nelson (voice)), Steve Coogan (Professor Flux (voice)), Jennifer Saunders (The Queen (voice)), Geoffrey Rush (Narrator (voice)), Steve Carell (Young Gru (voice)), Pierre Coffin (The Minions (voice)), Katy Mixon (Tina (voice)), Dave Rosenbaum (Fabrice (voice))