Onder andere met: Will Arnett (Batman / Bruce Wayne (voice)), Michael Cera (Robin / Dick Grayson (voice)), Rosario Dawson (Batgirl / Barbara Gordon (voice)), Ralph Fiennes (Alfred Pennyworth (voice)), Zach Galifianakis (Joker (voice)), Jenny Slate (Harley Quinn (voice)), Jason Mantzoukas (Scarecrow (voice)), Conan O'Brien (The Riddler (voice)), Doug Benson (Bane (voice)), Billy Dee Williams (Two-Face (voice)), Zoë Kravitz (Catwoman (voice)), Kate Micucci (Clayface (voice)), Riki Lindhome (Poison Ivy (voice)), Eddie Izzard (Voldemort (voice))